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KMID : 1094720220270040668
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 4 p.668 ~ p.677
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Elucidation of the Mechanism and Kinetics of Ultrasonic Extraction of Paclitaxel from Plant Cell Cultures of Taxus chinensis
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Kim Hak-Gyun
Kim Jin-Hyun
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Abstract
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In this study, the kinetics and mechanism of ultrasonic extraction of paclitaxel from Taxus chinensis were analyzed using ultrasonic cavitation bubbles and gas bubbles. The resulting paclitaxel yields (after one-time extraction) were 62?99% at ultrasonic power of 80?380 W in ultrasonic extraction and 61?76% at gas flow rate of 0.185?1.75 L/min in extraction using gas bubbles. These results show improvements from the yield obtained using conventional extraction (58%). As the ultrasonic power and gas flow rate increased, the extraction rate constant (3.0263?6.6028 mL/mg·min at 80?380 W and 2.9679?4.1067 mL/mg·min at 0.185?1.750 L/min), effective diffusion coefficient (12.4448 × 10?13?17.5691 × 10?13 m2/s at 80?380 W and 12.0788 × 10?13?16.1050 × 10?13 m2/s at 0.185?1.750 L/min), and mass transfer coefficient (2.5196 × 10?7?2.9750 × 10?7 m/s at 80?380 W and 2.3982 × 10?7?2.6411 × 10?7 m/s at 0.185?1.750 L/min) also increased. When ultrasonic extraction was executed using degassed solution (i.e., no cavitation), the paclitaxel yield was 51%, regardless of ultrasonic power; and the extraction rate constant, effective diffusion coefficient, and mass transfer coefficient were relatively small. This investigation of the ultrasonic extraction mechanism proved that cavitation bubbles themselves play a key role in promoting cell disruption, which has been indicated as improving the recovery efficiency of paclitaxel.
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KEYWORD
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paclitaxel, extraction, ultrasonic cavitation bubble, gas bubble, kinetics, mechanism
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